Module 3 – Stroma biology and organoid based co-cultures

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1. Why stroma is important
2. Stromal cells
3. Cancer associated fibroblasts (CAF)
4. Origin of CAFs in PDAC
5. CAF subtypes
6. Identification and functions of CAFs
7. The stroma as a drug target
8. Isolation of PSCs and CAFs co-cultures
9. Isolation of CAFs and PSCs from the tumor tissue
10. Isolation of CAFs – video
11. How to work with organoid based co-cultures
12. Importance of the co-culture system
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Module 3 Quiz
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4. Origin of CAFs in PDAC

Module 3 – Stroma biology and organoid based co-cultures 4. Origin of CAFs in PDAC

References:
1. Öhlund D, Handly-Santana A, Biffi G, Elyada E, Almeida AS, Ponz-Sarvise M, Corbo V, Oni TE, Hearn SA, Lee EJ, Chio II. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. Journal of Experimental Medicine. 2017 Mar 6;214(3):579-96.
2. Öhlund D, Elyada E, Tuveson D. Fibroblast heterogeneity in the cancer wound. Journal of Experimental Medicine. 2014 Jul 28;211(8):1503-23.
3. Sahai, E., Astsaturov, I., Cukierman, E. et al. A framework for advancing our understanding of cancer-associated fibroblasts. Nat Rev Cancer (2020).
4. Kalluri R. The biology and function of fibroblasts in cancer. ature Reviews Cancer. 2016 Sep;16(9):582. 
5. Biffi G, Oni TE, Spielman B, Hao Y, Elyada E, Park Y, Preall J, Tuveson DA. IL1-induced JAK/STAT signaling is antagonized by TGFβ to shape CAF heterogeneity in pancreatic ductal adenocarcinoma. Cancer discovery. 2019 Feb 1;9(2):282-301.

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