Module 3 – Stroma biology and organoid based co-cultures

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1. Why stroma is important
2. Stromal cells
3. Cancer associated fibroblasts (CAF)
4. Origin of CAFs in PDAC
5. CAF subtypes
6. Identification and functions of CAFs
7. The stroma as a drug target
8. Isolation of PSCs and CAFs co-cultures
9. Isolation of CAFs and PSCs from the tumor tissue
10. Isolation of CAFs – video
11. How to work with organoid based co-cultures
12. Importance of the co-culture system
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Module 3 Quiz
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11. How to work with organoid based co-cultures

Module 3 – Stroma biology and organoid based co-cultures 11. How to work with organoid based co-cultures

References:
1. Tsai S, McOlash L, Palen K, Johnson B, Duris C, Yang Q, Dwinell MB, Hunt B, Evans DB, Gershan J, James MA. Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models. BMC cancer. 2018 Dec;18(1):335
2. Öhlund D, Handly-Santana A, Biffi G, Elyada E, Almeida AS, Ponz-Sarvise M, Corbo V, Oni TE, Hearn SA, Lee EJ, Chio II. Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer. Journal of Experimental Medicine. 2017 Mar 6;214(3):579-96.

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