Module 2 – Overview and introduction to organoids

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1. Model systems in pancreatic cancer
2. 3D culture systems
3. Definition of terms
4. Organoid introduction
5. Importance of the matrix
6. Matrix influence on morphology and function
7. Stem cell contribution
8. Pancreatic organoids
9. Culture media composition
10. Immunocompromised mice
11. PDA progression
12. Murine organoid transplants
13. Mouse ODI to track immunoevolution of PDA
14. Changes in immune landscape
15. Establishment of human organoids
16. Pancreatic Ductal organoids
17. Different organoid technologies
18. Application: drug screening
Thank you
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5. Importance of the matrix

Module 2 – Overview and introduction to organoids 5. Importance of the matrix

References:
1. Simian M, Bissell MJ. Organoids: A historical perspective of thinking in three dimensions. J Cell Biol. 2017 Jan 2;216(1):31-40. doi: 10.1083/jcb.201610056. Epub 2016 Dec 28. Review. PubMed PMID: 28031422; PubMed Central PMCID: PMC5223613
2. Petersen OW, Rønnov-Jessen L, Howlett AR, Bissell MJ. Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9064-8. Erratum in: Proc Natl Acad Sci U S A 1993 Mar 15;90(6):2556. PubMed PMID: 1384042; PubMed Central PMCID: PMC50065.

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