Module 2 – Overview and introduction to organoids

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1. Model systems in pancreatic cancer
2. 3D culture systems
3. Definition of terms
4. Organoid introduction
5. Importance of the matrix
6. Matrix influence on morphology and function
7. Stem cell contribution
8. Pancreatic organoids
9. Culture media composition
10. Immunocompromised mice
11. PDA progression
12. Murine organoid transplants
13. Mouse ODI to track immunoevolution of PDA
14. Changes in immune landscape
15. Establishment of human organoids
16. Pancreatic Ductal organoids
17. Different organoid technologies
18. Application: drug screening
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18. Application: drug screening

Module 2 – Overview and introduction to organoids 18. Application: drug screening

References:
1. Baker LA, Tiriac H, Clevers H, Tuveson DA. Modeling pancreatic cancer with organoids. Trends Cancer. 2016 Apr;2(4):176-190. PubMed PMID: 27135056; PubMed Central PMCID: PMC4847151.
2. Francies HE, Barthorpe A, McLaren-Douglas A, Barendt WJ, Garnett MJ. Drug Sensitivity Assays of Human Cancer Organoid Cultures [published correction appears in Methods Mol Biol. 2018 Mar 27;:]. Methods Mol Biol. 2019;1576:339–351.
3. Ponz-Sarvise M, Corbo V, Tiriac H, Engle DD, Frese KK, Oni TE, Hwang CI, Öhlund D, Chio IIC, Baker LA, Filippini D, Wright K, Bapiro TE, Huang P, Smith P, Yu KH, Jodrell DI, Park Y, Tuveson DA. Identification of Resistance Pathways Specific to Malignancy Using Organoid Models of Pancreatic Cancer. Clin Cancer Res. 2019 Nov 15;25(22):6742-6755.

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